Microbial phylogenetic profiling with the Pacific Biosciences sequencing platform
نویسندگان
چکیده
منابع مشابه
Microbial phylogenetic profiling with the Pacific Biosciences sequencing platform
High-throughput sequencing of 16S rRNA gene amplicons has revolutionized the capacity and depth of microbial community profiling. Several sequencing platforms are available, but most phylogenetic studies are performed on the 454-pyrosequencing platform because its longer reads can give finer phylogenetic resolution. The Pacific Biosciences (PacBio) sequencing platform is significantly less expe...
متن کاملLong-read sequencing of the human cytomegalovirus transcriptome with the Pacific Biosciences RSII platform
Long-read RNA sequencing allows for the precise characterization of full-length transcripts, which makes it an indispensable tool in transcriptomics. The human cytomegalovirus (HCMV) genome has been first sequenced in 1989 and although short-read sequencing studies have uncovered much of the complexity of its transcriptome, only few of its transcripts have been fully annotated. We hereby presen...
متن کاملCorrigendum: Long-read sequencing of the human cytomegalovirus transcriptome with the pacific biosciences RSII platform
This corrects the article DOI: 10.1038/sdata.2017.194.
متن کاملMind the Gap: Upgrading Genomes with Pacific Biosciences RS Long-Read Sequencing Technology
Many genomes have been sequenced to high-quality draft status using Sanger capillary electrophoresis and/or newer short-read sequence data and whole genome assembly techniques. However, even the best draft genomes contain gaps and other imperfections due to limitations in the input data and the techniques used to build draft assemblies. Sequencing biases, repetitive genomic features, genomic po...
متن کاملMicrosatellite marker discovery using single molecule real-time circular consensus sequencing on the Pacific Biosciences RS.
Microsatellite sequences are important markers for population genetics studies. In the past, the development of adequate microsatellite primers has been cumbersome. However with the advent of next-generation sequencing technologies, marker identification in genomes of non-model species has been greatly simplified. Here we describe microsatellite discovery on a Pacific Biosciences single molecul...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Microbiome
سال: 2013
ISSN: 2049-2618
DOI: 10.1186/2049-2618-1-10